Dr Tsutomu Arie

Tsutomu Arie [Coordinator: North East Asia]
Position:Coordinator Region X: North East Asia

Laboratory of Plant Pathology, 
Institute of Agriculture, 
Tokyo University of Agriculture and Technology (TUAT)
3-5-8 saiwaicho, Fuchu 183-8509, Japan






Plant Pathology, Plant-Microbe Interactions, Fungal Biology, Molecular Biology, Evolution Biology, Plant Protection, Biological Control of Plant Diseases, Pesticide Science, and Integrated Disease Management

Dr. Arie is a plant pathologist especially studying on integrated management of plant diseases, and also on plant-soilborne pathogen interactions, and evolution and diversity of soilborne pathogens.  He has been collaborating with the researchers worldwide, for example, Cornell University, USA, Wageningen University, Netherlands, Can Tho University, Vietnum, La Molina Agronomy University, Peru, and Chapingo Autonomous University, Mexico.  He was awarded by the Pesticide Science Society of Japan in 1998 and by the Phytopathological Society of Japan in 2010.  He served as the secretary general of the Pesticide Science Society of Japan (2005–2008), and the Phytopathological Society of Japan (2013–2014).

Dr. Arie attended the International Plant Protection Congresses (IPPCs) two times at Rio de Janeiro in 1991 and at Honolulu in 2011 and gave presentations.

Dr. Arie is a professor in Graduate School of Agriculture of TUAT (Tokyo University of Agriculture and Technology) where he has been primarily responsible for the education of the graduate and undergraduate students so far.  From 2013 Dr. Arie has been served as a councilor of TUAT in charge of education and from 2015 he is serving as an advisor for the President of TUAT.

From 2015, two JICA (Japan International Cooperation Agency) projects will start to enhance Can Tho University, one of the most important Agronomy-oriented university in Vietnum, to be an internationally recognized university.  As the chairman of the supporting team composed of nine Japanese universities, Dr. Arie has been involved in designing the projects.  In the forthcoming project, he is scheduled to support for a research team on integrated management of insects and diseases in Mekong-Delta region.

Recent Major Publications

1. Arazoe T, Miyoshi K, Yamato T, Ohsato S, Arie T, Kuwata S (2015) Tailor-made CRISPR/Cas system for highly efficient targeted gene replacement in the rice blast fungus. Biotech Bioengineer DOI 10.1002/bit.25662
2. Arazoe T, Ogawa T, Miyoshi K, Yamato T, Ohsato S, Sakuma T, Yamamoto T, Arie T, Kuwata S (2015) Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus. Biotech Bioengineer 112:1335–42
3.  Inami K1, Kashiwa T, Kawabe M, Onokubo-Okabe A, Ishikawa N, Pérez ER, Hozumi T, Caballero LA, de Baldarrago FC, Roco MJ, Madadi KA, Peever TL, Teraoka T, Kodama M, Arie T (2014) The tomato wilt fungus Fusarium oxysporum f. sp. lycopersici shares common ancestors with nonpathogenic F. oxysporum isolated from wild tomatoes in the Peruvian Andes. Microbes Environ 29:200–210
4.  Kaneko I, Iyama-Kadono M, Togashi-Nishigata K, Yamaguchi I, Teraoka T, Arie T (2013) Heterotrimeric G protein β subunit GPB1 and MAP kinase MPK1 regulate hyphal growth and female fertility in Fusarium sacchari.  Mycoscience 54:148–157
5.  Kashiwa T, Inami K, Fujinaga M, Ogiso H, Yoshida T, Teraoka T, Arie T (2013) An avirulence gene homologue in the tomato wilt fungus Fusarium oxysporum f. sp. lycopersici race 1 functions as a virulence gene in the cabbage yellows fungus F. oxysporum f. sp. conglutinans. J Gen Plant Pathol DOI 10.1007/s10327-013-0471-5
6.  Yamazaki M, Yasuaki Morita, Kashiwa T, Teraoka T, Arie T (2013) Fusarium proliferatum, an additional bulb rot pathogen of Chinese chive. J Gen Plant Pathol DOI 10.1007/s10327-013-0473-3
7.  Inami K, Yoshioka-Akiyama C, Morita Y, Yamasaki M, Teraoka T, Arie T (2012) A genetic mechanism for emergence of Races in Fusarium oxysporum f. sp. lycopersici: Inactivation of avirulence gene AVR1 by transposon insertion.  PLoS ONE 7: e44101
8.  Ashizawa T, Takahashi M, Arai M, Arie T (2012) Rice false smut pathogen, Ustilaginoidea virens, invades through small gap at the apex of a rice spikelet before heading.  J Gen Plant Pathol 78:255–259
9.  Kato A, Miyake T, Nishigata K, Tateishi H, Teraoka T, Arie T (2012) Use of fluorescent proteins to visualize interactions between Gibberella fujikuroi, the ‘Bakanae disease’ pathogen, and the biocontrol agent Talaromyces sp. KNB-422.  J Gen Plant Pathol 78:54–61
10.  Kawabe M, Onokubo (Okabe) A, Arimoto Y, Yoshida T, Azegami K, Teraoka T, Arie T (2011) GMC oxidoreductase, a highly expressed protein in a potent biocontrol agent Fusarium oxysporum Cong:1-2, is dispensable for biocontrol activity.  J Gen Appl Microbiol 57:207–217
11.  Stewart JE, Kawabe M, Abdo Z, Arie T, Peever TL (2011) Contrasting codon usage patterns and purifying selection at the mating locus in putatively asexual Alternaria fungal species. PLoS ONE 6:e20083
12. Nakajima Y, Saitoh K-i, Arie T, Teraoka T, Kamakura T (2010) Expression specificity of CBP1 is regulated by transcriptional repression during vegetative growth of Magnaporthe oryzae. J Gen Appl Microbiol 56:437–445
13. Arie T (2010) Phylogeny and phytopathogenicity mechanisms of soilborne Fusarium oxysporum.  J Gen Plant Pathol 76:403–405 
14. Uehara T, Sugiyama S, Matsuura H, Arie T, Masuta C (2010) Resistant and susceptible responses in tomato to cyst nematode are differentially regulated by salicylic acid. Plant Cell Physiol 51:1524–1536 
15. Aoki K, …, Arie T(25the of the 31authors)… et al. (2010) Large-scale analysis of full-length cDNAs from the tomato (Solanum lycopersicum) cultivar Micro-Tom, a reference system for the Solanaceae genomics.  BMC Genomics 11:210
16. Inami K, Yoshioka C, Hirano Y, Kawabe M, Tsushima S, Teraoka T, Arie T (2010) Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici, and its races.  J Gen Plant Pathol 76:116–121
17. Izawa M, Takekawa O, Arie T, Teraoka T, Yoshida M, Kimura M, Kamakura T (2009) Inhibition of histone deacetylase causes reduction of appressorium formation in the rice blast fungus Magnaporthe oryzae.  J Gen Appl Microbiol 55:489–498
18. Hirano Y, Arie T (2009) Variation and phylogeny of Fusarium oxysporum isolates based on nucleotide sequence of polygalacturonase genes.  Microbe Environ 24:113–20 
19. Aoki N, Moriyama H, Kodama M, Arie T, Teraoka T, Fukuhara T (2009) A novel mycovirus associated with four double-stranded RNAs affects host fungal growth in Alternaria alternata. Virus Res 140: 179–187

Full list of publications can be seen at http://kenkyu-web.tuat.ac.jp/Profiles/4/0000317/profile.html